Rational substrate and enzyme engineering of transketolase for aromatics.
نویسندگان
چکیده
The uses of 3-formylbenzoic acid and 4-formylbenzoic acid as molecular probes along with previous and new transketolase mutants revealed the factors governing the rate of reaction between transketolase and aromatic aldehydes. The novel α,α-dihydroxyketones were produced at 15 to 30-fold higher yields and up to 250-fold higher specific activities with D469T TK when compared to those obtained for benzaldehyde.
منابع مشابه
Second generation engineering of transketolase for polar aromatic aldehyde substrates.
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عنوان ژورنال:
- Organic & biomolecular chemistry
دوره 10 45 شماره
صفحات -
تاریخ انتشار 2012